5-Vinyl-2'-deoxyuridine (5-VdU)

SKU: orb1734969

Description

5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

• Application Notes:
  DNA synthesis monitoring. ; Spectroscopic Propertie: max 292 nm, 9.2 L mmol-1 cm-1, (Methanol).

Key Properties

• CAS Number: 55520-67-7
• MW: Theoretical MW: 254.24 g/mol; Detected MW: 254.09 g/mol
• Purity: 98% (HPLC)
• Formula: C₁₁H₁₄N₂O₅
• SMILES: n1(cc(c(=O)[nH]c1=O)C=C)[C@H]1C[C@@H]([C@H](O1)CO)O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only