5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)

SKU: orb532331

Description

5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells. 5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine. The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to: introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes).

Images & Validation

• Application Notes:
  DNA synthesis monitoring (potentially). ; Spectroscopic Propertie: max 289 nm, 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 568.58 g/mol (free acid); Detected MW: 568.20 g/mol (free acid)
• Purity: 95% (HPLC)
• Formula: C₃₁H₂₈N₄O₇
• SMILES: c1(=O)[nH]c(=O)n(cc1C#CCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)CO

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only