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IKKα (phospho Thr23) rabbit pAb

SKU: orb764211
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Description

Rabbit polyclonal antibody to IKKalpha (phospho-Thr23)

Images & Validation

Tested ApplicationsELISA, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23. AA range:15-64
Molecular Weight85kD
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Anti-CHUK antibody, anti-IKKA antibody, anti-TCF16 antibody, anti-Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody, anti-I-kappa-B kinase alpha antibody, anti-IKK-A antibody, anti-IKK-alpha antibody, anti-IkBKA antibody, anti-IkappaB kinase antibody, anti-Conserved helix-loop-helix ubiquitous kinase antibody, anti-I-kappa-B ki antibody

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IKKα (phospho Thr23) rabbit pAb

Western Blot analysis of various cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000.

IKKα (phospho Thr23) rabbit pAb

Western Blot analysis of KB cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000.

IKKα (phospho Thr23) rabbit pAb

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IKK-alpha (Phospho-Thr23) Antibody. The picture on the right is blocked with the phospho peptide.

IKKα (phospho Thr23) rabbit pAb

Western blot analysis of lysates from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is blocked with the phospho peptide.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IKKα (phospho Thr23) rabbit pAb (orb764211)

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50 μl
$ 250.00
100 μl
$ 380.00
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