IgM-Reducing Assay Diluent

• Catalog Number: orb623163
• Category: Assays and Kits
• Description: IgM-Reducing Assay Diluent is a specially formulated additive to address IgM-mediated conjugate bridging interference in problematic serum and plasma samples tested in antibody sandwich ELISA formats. IgM-Reducing Assay Diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.
• Concentration: 1X
• Application notes: 1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT’s stabilizing blocker products, pipette 50 – 100 µL of Neptune Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The Assay Diluent is always added prior to adding the test and control serum/plasma samples to plate wells., 2. The control and test samples should be diluted to the proper well concentrations using one of ICT’s three different Sample Diluent products. Using an ICT Sample Diluent to dilute the serum and plasma samples will also serve to help minimize the degree of nonspecific IgG binding to the antigen coated and blocked ELISA plate wells. The test (sera and plasma) samples, diluted typically to 1:40 or greater in a sample diluent should be incubated in the antigen-coated ELISA plates for 30 – 60 minutes at room temperature or 37°C.
• Storage: 2-8°C
• Note: For research use only