IκB-α (phospho Tyr42) rabbit pAb Antibody

SKU: orb769255

Description

Rabbit polyclonal antibody to I kappa B-alpha (phospho-Tyr42)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr42. AA range:9-58
• Molecular Weight: 35
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-NFKBIA antibody, anti-IKBA antibody, anti-MAD3 antibody, anti-NFKBI antibody, anti-NF-kappa-B inhibitor alpha antibody, anti-I-kappa-B-alpha antibody, anti-IkB-alpha antibody, anti-IkappaBalpha antibody, anti-Major histocompatibility complex enhancer-binding protein MAD3 antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IkappaB-alpha (Phospho-Tyr42) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Tyr42) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night.