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Catalog Number | orb769259 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to I kappa B-beta (phospho-Thr19) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Thr19. AA range:4-53 |
MW | 37 |
Tested applications | ELISA, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-NFKBIB antibody, anti-IKBB antibody, anti-TRI Read more... |
Note | For research use only |
Western Blot analysis of 293 cells using Phospho-IκB-β (T19) Polyclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using IkappaB-beta (Phospho-Thr19) Antibody. The lane on the right is blocked with the phospho peptide.