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Human PDGF-AB
SKU: orb3002525
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Recombinant Human PDGF-AB (Cat#orb3002525) on SA Biosensor, can bind Recombinant Human PDGFRA (C-6His) (Cat#orb2994949) with an affinity constant of 84.5 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 13.43ng/ml.(Regularly tested) |
| Tag | Tag free |
| Molecular Weight | Predicted: 14.1&12.4 KDa. Observed: (14-16)&(17-19) KDa, reducing conditions |
| Expression Region | Ser87-Thr211&Ser82-Thr190 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | <0.1EU/μg as determined by LAL test. |
Storage & Handling
−| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
|---|---|
| Form/Appearance | Supplied as a 0.2 μm filtered solution of 4mM HCl. |
| Disclaimer | For research use only |
Alternative Names
−PDGF-AB
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Human PDGF-AB (orb3002525)
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