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Human PDGF-AB

SKU: orb3002525

Description

Recombinant Human PDGF-AB is produced by our E.coli expression system and the target gene encoding Ser87-Thr211&Ser82-Thr190 is expressed.

Images & Validation

Key Properties

Expression SystemE.coli
Biological ActivityBLI: Loaded Recombinant Human PDGF-AB (Cat#orb3002525) on SA Biosensor, can bind Recombinant Human PDGFRA (C-6His) (Cat#orb2994949) with an affinity constant of 84.5 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 13.43ng/ml.(Regularly tested)
TagTag free
Molecular WeightPredicted: 14.1&12.4 KDa. Observed: (14-16)&(17-19) KDa, reducing conditions
Expression RegionSer87-Thr211&Ser82-Thr190
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins<0.1EU/μg as determined by LAL test.

Storage & Handling

StorageStore at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Form/AppearanceSupplied as a 0.2 μm filtered solution of 4mM HCl.
DisclaimerFor research use only

Alternative Names

PDGF-AB

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Human PDGF-AB (orb3002525)

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