Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free, Low Endotoxin (MALS & SPR verified)

SKU: orb2938559

Description

Human ITGA4 & ITGB7 Heterodimer Protein, His Tag & Tag Free is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).

Images & Validation

• Application Notes:
  Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGA4) and 65 kDa, 70 kDa, 100-120 kDa (ITGB7) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: Integrin alpha 4 beta 7
• Reactivity: Human
• Tag: C-10×His | Native
• Molecular Weight: 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7)
• Expression Region: Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7)
• Purity: 90%
• Endotoxins: 0.01 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH 7.5
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Integrin alpha 4 beta 7

UniProt

P13612, P26010