Human IgG (H&L) Antibody Pre-Adsorbed

SKU: orb347184

Description

Human IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:2,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.12 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat Anti Human IgG Antibody Pre-Adsorbed, Goat Anti-Human IgG Antibody Pre-Adsorbed

ELISA Results of Goat Anti-Human IgG Antibody (MX Bv Ch Gt GP Ham Hs Ms Rb Rt Sh Serum Proteins) tested against purified Human IgG MX10. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n orb346219) [Red Line], Bovine IgG (p/n orb2652780) [Blue Line], Chicken IgG (orb2652774), Goat IgG (p/n orb2652767) [Purple Line], Guinea Pig IgG (p/n orb2652762), Hamster IgG (p/n orb2652759), Horse IgG (p/n orb2652757), Mouse IgG (p/n orb2652749), Rabbit IgG (p/n orb2652747), Rat IgG (p/n orb2652744), Sheep IgG (p/n orb2652741). The working dilution is 1:184500. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated (p/n orb347032) and TMB substrate (p/n orb348651).