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Catalog Number | orb49449 |
---|---|
Category | Proteins |
Description | Recombinant human IGFI protein |
Tag | tag free |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, pH 4.5. |
UniProt ID | P05019 |
Protein Sequence | Thr52-Ala118 |
Source | E.coli |
Expression System | E. coli |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 300mM NaAc, pH 6.5. |
Alternative names | InsulinLike Growth Factor I protein, IGFI protein, Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
9.5 kDa | |
Human IGF-I, His Tag, premium grade (orb1146986) is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019-1). |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE. |
Greater than 97% as determined by SDS-PAGE and HPLC. | |
7.7 kDa | |
E.Coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IGF1 is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
83.6 kDa and 22.8 kDa | |
Human Insulin R (28-944), His Tag (orb545724) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2). |
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