Human Anti-CS1 CAR-TF tag mRNA-LNP

• Catalog Number: orb2719924
• Category: Antibodies
• Description: CS1 (CD2 subset-1), also known as CD319 (SLAMF7), is a human natural killer (NK) cell receptor encoded by the SLAMF7 gene. This surface antigen is a robust marker of normal and malignant plasma cells in multiple myeloma. Human NK cells express two splice variants of CS1: CS1-S lacks an intracellular domain for activation, while CS1-L contains an intracellular domain and is therefore capable of activating NK cytotoxicity. CS1 is a homophilic receptor, and CS1-CS1 interaction leads to the activation of natural cytotoxicity of NK cells. Both isoforms of CS1 are membrane bound and expressed in NK cells. However, only the CS1-L isoform is expressed in B cells and regulates B cell proliferation and autocrine. This product is designed as a tool for the delivery and expression of human anti-CS1-TF CAR mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of human anti-CS1-TF CAR mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The human anti-CS1-TF CAR in this product is approximately 58 kD, consisting of human anti-CS1 scFv (single-chain variable fragment) linked to a second-generation CAR (chimeric antigen receptor) containing the CD8 hinge and transmembrane domain and 4-1BB and CD3ζ signaling domains. It contains an additional C-terminal TF tag (15 amino acids). The full-length amino acid sequence of this human anti-CS1-TF CAR mRNA-LNP product is available upon request.
• Species/Host: Human
• Conjugation: Unconjugated
• Form/Appearance: mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
• Application notes: Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only