Histone H1 (phospho Thr17) rabbit pAb

• Catalog Number: orb768586
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H1 (phospho-Thr17)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50
• MW: 31
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIST1H1B antibody, anti-H1F5 antibody, anti-H
• Note: For research use only

Immunofluorescence analysis of Hela cell. 1, Histone H1 (phospho Thr17) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE Monoclonal Antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of SKOV3 using p-Histone H1.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H1 (Phospho-Thr17) Antibody.

Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.