HIF-1α rabbit pAb

• Catalog Number: orb765387
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF-1 alpha
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
• Concentration: 1 mg/ml
• Dilution range: IF: 1:50-200 Western Blot: 1/500 - 1/2000.IP 1:200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 92-130
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-HIF1A antibody, anti-BHLHE78 antibody, anti-M
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from 1) Hela cell, 2) Hela cells knockdown by siRNA (F:GCCACAUUCACGUAUAUGATT, R:UCAUAUACGUGAAUGUGGCTT), (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.