HGH mRNA-LNP

SKU: orb2719932

Description

HGH (human growth hormone, or hGH) is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans. This protein is a member of the growth hormone/prolactin hormone family, encoded by the human GH1 gene, and plays an important role in growth control. HGH is secreted in the pituitary gland and regulated by the neurosecretory nucleus of the hypothalamus, and its secretion is determined by the balance of two other peptides, the peptides growth hormone-releasing hormone (GHRH or somatostatin) and growth hormone-inhibiting hormone (GHIH or somatostatin white) ). The study found that many mutations in the GH1 gene cause growth hormone deficiency, a condition characterized by slow growth and short stature. Mutations that prevent growth hormone production, such as a large deletion of the gene, cause isolated growth hormone deficiency type IA and severe growth failure in newborns. This product is designed as a tool for the delivery and expression of HGH (secreted) mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of HGH (secreted) mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The HGH (secreted) protein is about 22 kD, consisting of 191 amino acids (mature peptide only). The GenPept accession number is NP_000506.

Images & Validation

• Application Notes:
  Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage & Handling

• Storage: 4°C; ice
• Form/Appearance: mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
• Disclaimer: For research use only