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Item 1 of 4
Item 1 of 4
| Catalog Number | orb767281 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to HDAC5/9 (phospho-Ser259/220) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser259. AA range:225-274 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 121 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-HDAC5 antibody, anti-KIAA0600 antibody, anti- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of HepG2 cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of COLO HELA 293T cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1:2000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC5 (Phospho-Ser259) Antibody.
Western blot analysis of lysates from HepG2 cells, using HDAC5 (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.
