Goat anti-DBC1/p30 DBC Antibody

SKU: orb1523888

Description

DBC1/p30 DBC Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Between 873 and C-terminus
• Target: DBC1/p30 DBC
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

DBC-1; p30DBC; p30 DBC protein; p30 DBC; NET35; KIAA1967; Deleted in breast cancer gene 1 protein; DBIRD complex subunit KIAA1967; DBC1; DBC.1; cell division cycle and apoptosis regulator protein 2; cell cycle and apoptosis regulator protein 2; deleted in breast cancer 1

Detection of human DBC1/p30 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody orb1523888 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse DBC1/p30 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody orb1523888 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human DBC1/p30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody orb1523888 used for IP at 6 µg/mg lysate.

Detection of human DBC1/p30 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BC1/p30 (Cat. No. orb1523888) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse DBC1/p30 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-BC1/p30 (Cat. No. orb1523888) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

DBC1/p30 DBC

UniProt

Q8N163

RefSeq (Protein):NP_066997.3