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Catalog Number | orb18804 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to BCL2L11 |
Target | BIM |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | FNAYYARRLEK |
RRID | AB_10749720 |
MW | 18.5; 12.3; 9.08 |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:16000, WB: 0.5-2 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 23kDa band observed in K562 lysates (predicted MW of 19, 9, 12kDa according to NP_619528; NP_996886; NP_619529). Recommended for use at 0.5-2 μg/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti BIM antibody |
Research Area | Apoptotic, Cancer, Epigenetics, Neuroscience, Sign Read more... |
Note | For research use only |
Entrez | 10018 |
Expiration Date | 12 months from date of receipt. |
orb18804 staining (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.