Goat anti-BIM Antibody

SKU: orb18804

Description

Goat polyclonal antibody to BCL2L11

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-2 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 23kDa band observed in K562 lysates (predicted MW of 19, 9, 12kDa according to NP_619528; NP_996886; NP_619529). Recommended for use at 0.5-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BIM
• Molecular Weight: 18.5; 12.3; 9.08
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti BIM antibody

orb18804 staining (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

BIM

NCBI Gene ID

10018