Glypican-3 rabbit pAb

• Catalog Number: orb766811
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Glypican-3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human GPC3. AA range:461-510
• MW: 70
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-GPC3 antibody, anti-OCI5 antibody, anti-Glypi
• Note: For research use only

Western Blot analysis of HepG2 cells using Glypican-3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100.

Western blot analysis of lysate from HepG2 cells, using GPC3 Antibody.