Gab 2 (phospho Ser159) rabbit pAb

• Catalog Number: orb770982
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Gab 2 (phospho-Ser159)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human GRB2 around the phosphorylation site of Ser159. AA range:125-174
• MW: 100
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-GAB2 antibody, anti-KIAA0571 antibody, anti-G
• Note: For research use only

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GRB2 (Phospho-Ser159) Antibody.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using GRB2 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using GRB2 (Phospho-Ser159) Antibody. The lane on the right is blocked with the phospho peptide.