FOXQ1 Antibody

SKU: orb2634788

Description

DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Images & Validation

• Tested Applications: ELISA, FACS, WB
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the FOXQ1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PCRP-FOXQ1-2D2
• Immunogen: Recombinant full-length human FOXQ1 protein was used as the immunogen for the FOXQ1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using FOXQ1 antibody (clone PCRP-FOXQ1-2D2). These results demonstrate the foremost specificity of the PCRP-FOXQ1-2D2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FACS staining of PFA-fixed human HeLa cells with FOXQ1 antibody (blue, clone PCRP-FOXQ1-2D2), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free FOXQ1 antibody (clone PCRP-FOXQ1-2D2) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q9C009