FOXP1 Antibody [RAB-S37], Rabbit IgG

• Catalog Number: orb1671519
• Category: Antibodies
• Description: FOXP1 Antibody [RAB-S37], Rabbit IgG
• Target: FOXP1
• Clonality: Recombinant
• Isotype: IgG kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: batch dependent
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP1 protein under non-denaturing conditions.
• UniProt ID: Q9H334
• Tested applications: ELISA, FC, IF
• Antibody Type: Primary Antibody
• Clone Number: RAB-S37
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MFH, Forkhead box protein P1, Mac-1-regulated fork
• Note: For research use only

Western Blot using anti-FOXP1 antibody. Human skeletal muscle tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion at 0.003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the Anti-FOXP1 antibody. Paraformaldehyde fixed MCF7 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with anti-FOXP1. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permewith 0.15% Triton stained with the chimeric r version (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671519- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.