EFP rabbit pAb Antibody

SKU: orb770422

Description

Rabbit polyclonal antibody to EFP

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ZNF147. AA range:201-250
• Molecular Weight: 71
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-TRIM25 antibody, anti-EFP antibody, anti-RNF147 antibody, anti-ZNF147 antibody, anti-E3 ubiquitin/ISG15 ligase TRIM25 antibody, anti-Estrogen-responsive finger protein antibody, anti-RING finger protein 147 antibody, anti-Tripartite motif-containing protein 25 antibody, anti-Ubiquitin/ISG15-conjugating enzyme T antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from K562 cells, using ZNF147 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:10000.