Dok-2 (phospho Tyr299) rabbit pAb Antibody

SKU: orb764174

Description

Rabbit polyclonal antibody to Dok-2 (phospho-Tyr299)

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Monkey, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human p56 Dok-2 around the phosphorylation site of Tyr299. AA range:266-315
• Molecular Weight: 48
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-DOK2 antibody, anti-Docking protein 2 antibody, anti-Downstream of tyrosine kinase 2 antibody, anti-p56(dok-2) antibody

Western Blot analysis of various cells using Phospho-Dok-2 (Y299) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p56 Dok-2 (Phospho-Tyr299) Antibody.

Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ml 15', Jurkat cells treated with insulin 0.01U/ml 15' and 293 cells treated with serum 20% 15', using p56 Dok-2 (Phospho-Tyr299) Antibody. The lane on the right is blocked with the phospho peptide.