Dnmt3b rabbit pAb

SKU: orb765064

Description

Rabbit polyclonal antibody to DNMT3B

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Gallus, Human

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DNMT3B. AA range:1-50
• Molecular Weight: 96
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-DNMT3B antibody, anti-DNA (cytosine-5)-methyltransferase 3B antibody, anti-Dnmt3b antibody, anti-DNA methyltransferase HsaIIIB antibody, anti-DNA MTase HsaIIIB antibody, anti-M.HsaIIIB antibody

Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 Monoclonal Antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Immunofluorescence analysis of human-breast-cancer tissue. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-breast-cancer tissue. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-liver-cancer tissue. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.