DNA Ligase IV rabbit pAb Antibody

SKU: orb768988

Description

Rabbit polyclonal antibody to DNA Ligase IV

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DNA Ligase 4. AA range:616-665
• Molecular Weight: 133
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-LIG4 antibody, anti-DNA ligase 4 antibody, anti-DNA ligase IV antibody, anti-Polydeoxyribonucleotide synthase [ATP] 4 antibody

Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.