DNA Ligase I rabbit pAb

SKU: orb765056

Description

Rabbit polyclonal antibody to DNA Ligase I

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DNL1. AA range:111-160
• Molecular Weight: 133
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-LIG1 antibody, anti-DNA ligase 1 antibody, anti-DNA ligase I antibody, anti-Polydeoxyribonucleotide synthase [ATP] 1 antibody

Western Blot analysis of various cells using DNA Ligase I Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29 cells, using DNL1 Antibody. The lane on the right is blocked with the synthesized peptide.