DMRTA2 Antibody

• Catalog Number: orb1265253
• Category: Antibodies
• Description: DMRTA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Zebrafish
• Isotype: Rabbit Ig
• Immunogen: This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: DMRTA2
• UniProt ID: Q96SC8
• NCBI: Q96SC8
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Doublesex- and mab-3-related transcription factor
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35 ug/lane).

Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.