Desmin Antibody

• Catalog Number: orb1151455
• Category: Antibodies
• Description: Mouse monoclonal antibody to Desmin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Feline, Human
• Immunogen: Recombinant full-length human desmin protein was used as the immunogen for the Desmin antibody.
• UniProt ID: P17661
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Application notes: Optimal dilution of the Desmin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: DES/3255
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Desmin antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human cardiac muscle tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skeletal muscle with Desmin antibody (clone DES/3255) at 2 ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog smooth muscle tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat smooth muscle tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE horse smooth muscle tissue withe Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cow small intestine tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Desmin antibody (clone DES/3255) as confirmation of integrity and purity.