DDAO Phosphate

SKU: orb532194

Description

DDAO-phosphate is a long-wavelength, phosphatase substrate for both solution-based assays and the amplified detection of specific targets on blots. Hydrolysis of the phosphate bond yields a brightly red-fluorescent product with absorption/emission maxima of 646/659 nm that is efficiently excited by the 633 nm spectral line of the He-Ne laser. The shift of emission maxima between DDAO-phosphate and the dephosphorylated product is larger than 200 nm and permits both species to be easily distinguished. DDAO phosphate has good water solubility, a low KM and a high turnover rate.

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λexc 477 nm, λem 628 nm, ε 26.0 L mmol-1 cm-1(50 mM Potassium Phosphate pH 7.0).

Key Properties

• CAS Number: 500883-59-0
• MW: Theoretical MW: 388.14 g/mol (free acid); Detected MW: 386.98 g/mol (free acid)
• Purity: > 95% (HPLC)
• Formula: C₁₅H₁₂Cl₂NO₅P
• SMILES: c1cc2c(cc1OP(=O)(O)O)C(C1=C(C(=O)C(=CC1=N2)Cl)Cl)(C)C

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only