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Catalog Number | orb532194 |
---|---|
Category | Tools |
Description | DDAO-phosphate is a long-wavelength, phosphatase substrate for both solution-based assays and the amplified detection of specific targets on blots. Hydrolysis of the phosphate bond yields a brightly red-fluorescent product with absorption/emission maxima of 646/659 nm that is efficiently excited by the 633 nm spectral line of the He-Ne laser. The shift of emission maxima between DDAO-phosphate and the dephosphorylated product is larger than 200 nm and permits both species to be easily distinguished. DDAO phosphate has good water solubility, a low KM and a high turnover rate. |
CAS Number | 500883-59-0 |
Form/Appearance | solid |
Purity | > 95% (HPLC) |
MW | Theoretical MW: 388.14 g/mol (free acid); Detected MW: 386.98 g/mol (free acid) |
SMILES | c1cc2c(cc1OP(=O)(O)O)C(C1=C(C(=O)C(=CC1=N2)Cl)Cl)(C)C |
Formula | C15H12Cl2NO5P |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Spectroscopic Propertie: λexc 477 nm, λem 628 nm, ε 26.0 L mmol-1 cm-1(50 mM Potassium Phosphate pH 7.0). |
Expiration Date | 12 months from date of receipt. |
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