DCI Antibody

• Catalog Number: orb1262519
• Category: Antibodies
• Description: DCI Antibody
• Target: ECI1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This DCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-302 amino acids from the C-terminal region of human DCI.
• UniProt ID: P42126
• MW: 33 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Enoyl-CoA delta isomerase 1, mitochondrial,2-trans
• Note: For research use only
• NCBI: P42126

Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with DCI Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.