Dbf4 rabbit pAb

• Catalog Number: orb765023
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Dbf4
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Monkey, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 77
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-DBF4 antibody, anti-ASK antibody, anti-DBF4A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Dbf4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from NIH/3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30', using DBF4 Antibody. The lane on the right is blocked with the synthesized peptide.