DAPK3 (phospho Thr265) rabbit pAb

SKU: orb767842

Description

Rabbit polyclonal antibody to DAPK3 (phospho-Thr265)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
• Molecular Weight: 52
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-DAPK3 antibody, anti-ZIPK antibody, anti-Death-associated protein kinase 3 antibody, anti-DAP kinase 3 antibody, anti-DAP-like kinase antibody, anti-Dlk antibody, anti-MYPT1 kinase antibody, anti-Zipper-interacting protein kinase antibody, anti-ZIP-kinase antibody

Immunofluorescence analysis of Hela cell. 1, DAPK3 (phospho Thr265) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). α-tubulin Monoclonal Antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of HuvEc cells using Phospho-DAPK3 (T265) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK3 (Phospho-Thr265) Antibody.

Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) Antibody. The picture on the right is blocked with the phospho peptide.