CYP27A1 rabbit pAb

• Catalog Number: orb764971
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CYP27A1
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cytochrome P450 27A1. AA range:101-150
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 60
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CYP27A1 antibody, anti-CYP27 antibody, anti-S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using CYP27A1 Polyclonal Antibody diluted at 1:1000.

Western blot analysis of lysates from HeLa cells, using Cytochrome P450 27A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).