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| Catalog Number | orb770808 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Cyclin H (phospho-Thr315) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin H around the phosphorylation site of Thr315. AA range:274-323 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 34 36 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-CCNH antibody, anti-Cyclin-H antibody, anti-M Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin H (Phospho-Thr315) Antibody.
Western blot analysis of lysates from HepG2 cells, Jurkat cells, HUVEC cells and A549 cells, using Cyclin H (Phospho-Thr315) Antibody. The lane on the right is blocked with the phospho peptide.
