Cyclin H (phospho Thr315) rabbit pAb

• Catalog Number: orb770808
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin H (phospho-Thr315)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin H around the phosphorylation site of Thr315. AA range:274-323
• MW: 34 36
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CCNH antibody, anti-Cyclin-H antibody, anti-M
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin H (Phospho-Thr315) Antibody.

Western blot analysis of lysates from HepG2 cells, Jurkat cells, HUVEC cells and A549 cells, using Cyclin H (Phospho-Thr315) Antibody. The lane on the right is blocked with the phospho peptide.