Cyclin D1 (phospho Thr286) rabbit pAb

• Catalog Number: orb764301
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin D1 (phospho-Thr286)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286. AA range:246-295
• MW: 33
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CCND1 antibody, anti-BCL1 antibody, anti-PRAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.