Connexin 43 (phospho Ser368) rabbit pAb

SKU: orb764166

Description

Rabbit polyclonal antibody to Connexin 43 (phospho-Ser368)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Connexin 43 around the phosphorylation site of Ser367. AA range:332-381
• Molecular Weight: 43
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-GJA1 antibody, anti-GJAL antibody, anti-Gap junction alpha-1 protein antibody, anti-Connexin-43 antibody, anti-Cx43 antibody, anti-Gap junction 43 kDa heart protein antibody

Western Blot analysis of various cells using Phospho-Connexin 43 (S368) Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HuvEc cells using Phospho-Connexin 43 (S368) Polyclonal Antibody diluted at 1:2000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43 (Phospho-Ser367) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with PMA 200ng/ml 10', using Connexin 43 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.