Claudin-5 (phospho Tyr217) rabbit pAb

• Catalog Number: orb770275
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Claudin-5 (phospho-Tyr217)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Claudin 5 around the phosphorylation site of Tyr217. AA range:169-218
• MW: 23
• Tested applications: ELISA, IHC-P, WB
• Dilution range: WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CLDN5 antibody, anti-AWAL antibody, anti-TMVC
• Note: For research use only

Immunohistochemistry analysis of paraffin-embedded human brain, using Claudin 5 (Phospho-Tyr217) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night.