Che-1 rabbit pAb

SKU: orb764842

Description

Rabbit polyclonal antibody to Che-1

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human AATF. AA range:10-59
• Molecular Weight: 63
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-AATF antibody, anti-CHE1 antibody, anti-DED antibody, anti-HSPC277 antibody, anti-Protein AATF antibody, anti-Apoptosis-antagonizing transcription factor antibody, anti-Rb-binding protein Che-1 antibody

Western Blot analysis of various cells using Che-1 Polyclonal Antibody.

Western blot analysis of lysates from HeLa cells, HepG2 cells, COLO205 cells, and HUVEC cells, using AATF Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).