C/EBP α (phospho Ser21) rabbit pAb Antibody

SKU: orb764343

Description

Rabbit polyclonal antibody to C/EBP alpha (phospho-Ser21)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
• Molecular Weight: 45
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CEBPA antibody, anti-CCAAT/enhancer-binding protein alpha antibody, anti-C/EBP alpha antibody

Western blot analysis of lysates from Mouse-liver, Rat-liver, Hela cells treated with 0.01U/ml 30min, using C/EBP α was diluted at 1:10000, 37° 1hour.

Western Blot analysis of various cells using Phospho-C/EBP α (S21) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C/EBP-alpha (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.