Cdc16 (phospho Ser560) rabbit pAb

SKU: orb770774

Description

Rabbit polyclonal antibody to Cdc16 (phospho-Ser560)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDC16/APC6 around the phosphorylation site of Ser560. AA range:526-575
• Molecular Weight: 72
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CDC16 antibody, anti-ANAPC6 antibody, anti-Cell division cycle protein 16 homolog antibody, anti-Anaphase-promoting complex subunit 6 antibody, anti-APC6 antibody, anti-CDC16 homolog antibody, anti-CDC16Hs antibody, anti-Cyclosome subunit 6 antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC16/APC6 (Phospho-Ser560) Antibody.

Immunofluorescence analysis of HeLa cells, using CDC16/APC6 (Phospho-Ser560) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using CDC16/APC6 (Phospho-Ser560) Antibody. The picture on the right is blocked with the phospho peptide.