CD21 Antibody

• Catalog Number: orb639640
• Category: Antibodies
• Description: Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A partial protein corresponding to amino acids 142-240 was used as the immunogen for the recombinant CD21 antibody.
• UniProt ID: P20023
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CR2/3124R
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant CD21 antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R); Red=isotype control, Blue= recombinant CD21 antibody.

IHC staining of FFPE human tonsil with recombinant CD21 antibody (clone CR2/3124R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone CR2/3124R) as confirmation of integrity and purity.