CD15 rabbit pAb

• Catalog Number: orb768288
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD15
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human FucT-IV. AA range:22-71
• MW: 46kD
• Tested applications: ELISA, IHC-P
• Dilution range: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-FUT4 antibody, anti-ELFT antibody, anti-FCT3A
• Note: For research use only

Fluorescence multiplex immunohistochemical analysis of normal human gastric cancer tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CD15 YT0726 (1/50 dilution), CD11b YT5923 (1/50 dilution), CD14 YM6145 (1/50 dilution), each using a separate fluorescent tyramide signal amplification system.Sodium citrate antigen retrieval (Immunoway YS0002, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.DAPI (dark blue) was used as a nuclear counter stain.

Immunohistochemistry analysis of FucT-IV antibody in paraffin-embedded human breast carcinoma tissue.