CD15 rabbit pAb

SKU: orb768288

Description

Rabbit polyclonal antibody to CD15

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution range: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FucT-IV. AA range:22-71
• Molecular Weight: 46kD
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-FUT4 antibody, anti-ELFT antibody, anti-FCT3A antibody, anti-Alpha-(1 antibody, anti-3)-fucosyltransferase antibody, anti-ELAM-1 ligand fucosyltransferase antibody, anti-Fucosyltransferase 4 antibody, anti-Fucosyltransferase IV antibody, anti-Fuc-TIV antibody, anti-FucT-IV antibody, anti-Galactoside 3-L-fucosyltransferase antibody

Fluorescence multiplex immunohistochemical analysis of normal human gastric cancer tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CD15 YT0726 (1/50 dilution), CD11b YT5923 (1/50 dilution), CD14 YM6145 (1/50 dilution), each using a separate fluorescent tyramide signal amplification system.Sodium citrate antigen retrieval (Immunoway YS0002, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.DAPI (dark blue) was used as a nuclear counter stain.

Immunohistochemistry analysis of FucT-IV antibody in paraffin-embedded human breast carcinoma tissue.