CD11b rabbit pAb

• Catalog Number: orb771548
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD11b
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Synthetic peptide from human protein at AA range: 280-360
• Concentration: 1 mg/ml
• Dilution range: IHC-p 1:50-200, ELISA 1:10000-20000
• Conjugation: Unconjugated
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-Integrin alpha-M (CD11 antigen-like family me
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescence multiplex immunohistochemical analysis of normal human gastric cancer tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CD15 YT0726 (1/50 dilution), CD11b YT5923 (1/50 dilution), CD14 YM6145 (1/50 dilution), each using a separate fluorescent tyramide signal amplification system.Sodium citrate antigen retrieval (Immunoway YS0002, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.DAPI (dark blue) was used as a nuclear counter stain.

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.