CCL2 mRNA-LNP

• Catalog Number: orb2719981
• Category: Proteins
• Description: CCL2 (Chemokine (C-C motif) ligand 2), commonly known as monocyte chemoattractant protein-1 (MCP-1), belongs to a family of secreted proteins involved in immune regulatory and inflammatory processes. Mature wild-type CCL2 protein has a full length of 76 amino acids, and its N-terminal residues are crucial in activating the CCL2 signaling pathway. The CCL2 mutant (9-76 amino acids), also known as 7ND, is a deletion of the first 8 residues of wild-type CCL2, which can bind to the C-C chemokine receptors CCR2 and CCR4 on the surface of monocytes, but cannot activate the down-regulated pathway. Therefore, the CCL2 mutant can be used as an inhibitory form of wild-type CCL2 to block the C-C chemokine receptors of CCL2 and inhibit unwanted monocyte/macrophage recruitment. This product is designed as a tool for the delivery and expression of the mature wild-type CCL2 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of CCL2 mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The GenPept accession number for CCL2 is NP_002973.
• Form/Appearance: mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
• Application notes: Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
• Storage: 4°C; ice
• Note: For research use only