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| Catalog Number | orb1260834 |
|---|---|
| Category | Antibodies |
| Description | CBX5 Antibody |
| Target | CBX5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CBX5 (NP_001120794.1). |
| UniProt ID | P45973 |
| MW | Observed: 22kDa |
| Tested applications | IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CBX5, HP1, HP1A, HEL25 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of extracts of various cell lines, using CBX5 antibody (orb1260834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat hippocampus using CBX5 antibody (orb1260834) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human brain astrocytoma using CBX5 antibody (orb1260834) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using CBX5 antibody (orb1260834) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using CBX5 antibody (orb1260834) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CBX5 antibody (orb1260834) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CBX5 antibody (orb1260834) at dilution of 1:100 (40x lens).
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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