Caspase-1 (phospho Ser376) rabbit pAb

SKU: orb770554

Description

Rabbit polyclonal antibody to Caspase-1 (phospho-Ser376)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 1 around the phosphorylation site of Ser376. AA range:342-391
• Molecular Weight: 29
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CASP1 antibody, anti-IL1BC antibody, anti-IL1BCE antibody, anti-Caspase-1 antibody, anti-CASP-1 antibody, anti-Interleukin-1 beta convertase antibody, anti-IL-1BC antibody, anti-Interleukin-1 beta-converting enzyme antibody, anti-ICE antibody, anti-IL-1 beta-converting enzyme antibody, anti-p45 antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 1 (Phospho-Ser376) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain, using Caspase 1 (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells, using Caspase 1 (Phospho-Ser376) Antibody. The lane on the right is blocked with the phospho peptide.