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Catalog Number | orb764709 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Calregulin |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Monkey, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Immunogen | The antiserum was produced against synthesized peptide derived from human CALR. AA range:21-70 |
MW | 48 |
Tested applications | ELISA, IF, IHC-P, WB |
Dilution range | WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-CALR antibody, anti-CRTC antibody, anti-Calre Read more... |
Note | For research use only |
Immunofluorescence analysis of Hela cell. 1, Calregulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Western Blot analysis of various cells using Calregulin Polyclonal Antibody.
Immunofluorescence analysis of NIH/3T3 cells, using CALR Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using CALR Antibody. The picture on the right is blocked with the synthesized peptide.
FC, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |