CA XII rabbit pAb

SKU: orb770423

Description

Rabbit polyclonal antibody to CA XII

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CA12. AA range:231-280
• Molecular Weight: 39
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CA12 antibody, anti-Carbonic anhydrase 12 antibody, anti-Carbonate dehydratase XII antibody, anti-Carbonic anhydrase XII antibody, anti-CA-XII antibody, anti-Tumor antigen HOM-RCC-3.1.3 antibody

Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1:2000.

Western blot analysis of lysates from MCF-7 cells, using CA12 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from COLO205 cells using CA12 antibody.

Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).