c-Kit rabbit pAb

• Catalog Number: orb768898
• Category: Antibodies
• Description: Rabbit polyclonal antibody to c-Kit
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human KIT. AA range:906-955
• MW: 117
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-KIT antibody, anti-SCFR antibody, anti-Mast/s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 453 cells using c-Kit Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from COLO205 cells, using KIT Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using KIT antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).