Bub3 rabbit pAb

• Catalog Number: orb764675
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Bub3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human BUB3. AA range:279-328
• MW: 40
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-BUB3 antibody, anti-Mitotic checkpoint protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Bub3 Polyclonal Antibody.

Western Blot analysis of 293 cells using Bub3 Polyclonal Antibody.

Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using BUB3 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).